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For subcutaneous administration, 250-500 mcg per day represents the standard range, with some protocols using 300 mcg as a target dose

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The supernatant was centrifuged again at 10,000 rcf for 5 min at 4 C

The 250500 mcg subcutaneous range used in current research contexts sits below the 1 mg clinical trial dose, partly because subcutaneous bioavailability differs from the oral administration used in some trial arms